Recombinant Mouse FGF-2/FGF basic/bFGF Protein is produced by E. coli expression system. The target protein is expressed with sequence (Met1-Ser154) of mouse FGF-2/FGF basic/bFGF (Accession #P15655).
FGF basic is one of 22 mitogenic proteins of the FGF family, which show 35-60% amino acid conservation.Unlike other FGFs, FGF acidic and basic lack signal peptides and are secreted by an alternate pathway. The 17kDa mouse sequence has 98% aa identity with rat, and 95% identity with human, bovine, and sheep FGF basic.Binding of FGF to heparin or cell surface HSPG is necessary for binding, dimerization and activation of tyrosinekinase FGF receptors. FGF basic binds other proteins, polysaccharides and lipids with lower affinity. Expressionof FGF basic is nearly ubiquitous but disruption of the mouse FGF basic gene gives a relatively mild phenotype,suggesting compensation by other FGF family members. FGF basic modulates such normal processes asangiogenesis, wound healing and tissue repair, embryonic development and differentiation, neuronal functionand neural degeneration. Transgenic overexpression of FGF basic results in excessive proliferation andangiogenesis is reminiscent of a variety of pathological conditions.
|Purity||> 95% by SDS-PAGE.|
|Endotoxin||< 1 EU/μg of the protein by LAL method.|
|Formulation||Lyophilized from a 0.2 μm filtered solution of 20mM PB, 400mM NaCl, pH 7.0.|
|Reconstitution||Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.|
|Storage Conditions||Store the lyophilized protein at -20°C to -80 °C for long term. After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.|
$520.00 – $750.00