Recombinant Human AFP/Alpha-Fetoprotein Protein is produced by Human Cells expression system. The target protein is expressed with sequence (Arg19-Val609) of human AFP/Alpha-Fetoprotein (Accession #P02771) fused with a 6×His tag at the C-terminus.
Alpha-fetoprotein (AFP) is classified as a member of the albuminoid gene superfamily consisting of albumin, AFP, vitaminD(Gc) protein, and alpha-albumin. AFP is a glycoprotein of 591 amino acids and a carbohydrate moiety. AFP is a majorplasma protein produced by the yolk sac and the liver during fetal development. It is thought to be the fetal form of serumalbumin. AFP binds to copper, nickel, fatty acids and bilirubin and is found in monomeric, dimeric and trimeric forms. AFPis one of the several embryo-specific proteins and is adominant serum protein as early in human embryonic life as onemonth, when albumin and transferrin are present in relatively small amounts. It is first synthesized in the human by theyolk sac and liver (1-2 months) and subsequently predominantly in the liver. A small amount of AFP is produced by the GItract of the human conceptus. It has been proved that AFP may reappear in the serum in elevated amounts in adult life inassociation with normal restorative processes and with malignnt growth. Alpha-fetoprotein (AFP) is a specific marker forhepatocellular carcinoma (HCC), teratoblastomas, and neural tube defect (NTD).
|Routing||6×His tag at the C-terminus|
|Purity||> 95% by SDS-PAGE.|
|Endotoxin||< 1 EU/μg of the protein by LAL method.|
|Formulation||Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.|
|Reconstitution||Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.|
|Storage Conditions||Store the lyophilized protein at -20°C to -80 °C for long term. After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.|
$1,300.00 – $1,870.00