293T mCTLA4 (CD152) Description
HEK 293T cells stably expressing exogenous mouse CTLA-4 gene.
Background
Cytotoxic T lymphocyte-associated protein 4 (CTLA4), also named CD152, is a membrane protein receptor acting as a negative regulator of T cell responsive when bound to its ligands on the surface of antigen-presenting cells, including CD80 and CD86. The dysfunction and overactivation of CTLA4 has been proved to lead to immune dysfunction syndrome and tumor immunological escape, its monoclonal antibody, Ipilmumab, is the first approved immune checkpoint blockade therapy and has been proved to be a potential therapeutical strategy for cancer patient.
Alternative Nomenclature
Also known as: CD152
Cell Line Characterization
Recombinant Cell Line Profile
| Catalog #: | C2082 |
| Size: | 1 Vial (5M Cells) |
|---|---|
| Species: | Human |
| Cell Line ID: | 293T/mCTLA4 |
| Cell Type: | Human embryonic kidney |
| Selection Marker: | Puromycin (0.8ug/ml) |
| Application: | Drug screening and biological assays |
| Morphology: | Fibroblastoid cells growing adherently as monolayer |
| Medium: | RPMI 1640 + 10% FBS + 0.8ug/ml Puromycin |
| Subculture: | Seed out at ca. 2-3 x 106 cells/80 cm2; split confluent culture 1:4 - 1:5 every 2-3 days |
| Incubation: | 37 °C with 5% CO2 |
| storage: | Frozen in liquid nitrogen with 70% medium + 20% FBS + 10% DMSO |
| Doubling Time: | 24-30 hours |
| Biosafety Level: | 2 |
| References: | Cancer Immunol Immunother. 2018 Jan;67(1):47-60. |
Resuscitation Protocol
Thaw the vial as soon as possible upon receipt to ensure its viability.
1. Thaw the frozen vial in a 37°C water bath for 1-2 minutes.
2. Transfer the vial into biosafety cabinet. Wipe the surface with 70% ethanol and drop the cell suspension gently into a centrifuge tube containing 9.0 mL complete culture medium.
3. Spin at ~ 125 x g, for 5- 7 minutes at room temperature.
4. Resuspend cell pellet with the appropriate volume of complete medium and transfer the cell suspension into a T25 culture flask.
5. Incubate the culture at 37°, 5% CO2 incubator or refer to the specific culture condition, if possible.
Cell Line Limited Use License
FOR IN VITRO REASEARCH USE ONLY. NOT FOR THERAPEUTIC OR DIAGNOSTIC USE. BY ACCEPTING THIS PRODUCT, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT.
This product may not be resold or transferred by the recipient and may be used only by the recipient and its affiliate (as defined below), in their facility and only for (1) research use, (2) drug discovery and development of biologic drugs, (3) quality assurance testing of biologic drugs, and (4) assays for biologic drugs. No other use, especially any commercial use, is allowed. “Commercial use” means any and all uses of this product by recipient for monetary or other consideration, including providing a service, supplying information or data to unaffiliated third parties, and resale of this product for any use. Recipient has no right to modify, derivatize, genetically engineer, reverse engineer, duplicate or otherwise create variations of the Cells.
Crown Bioscience shall have the sole ownership, interest, and tittle in any materials it transfers under this Agreement. The recipient(s) shall not transfer any materials transferred by Crown Bioscience under this Agreement to any third parties without Crown Bioscience’s prior written consent.
CROWN BIOSCIENCE MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING AS TO MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THIS PRODUCT. The terms of this agreement shall be governed under the laws of the State of California, USA.
“Affiliate” shall mean, with respect to an entity, any other entity that directly or indirectly controls, is controlled by, or is under common control with, the entity. For the purpose of this definition of Affiliate, “control” means the ownership and voting control of more than fifty percent (50%) of the outstanding voting securities or interest in capital or profits of an entity, or the right to direct or control the management or affairs of such entity by contract or similar arrangement.
To obtain a COMMERCIAL USE License please contact Crown Bioscience, Inc.
