BTLA, also known as CD272, is a ligand for TNFRSF14 (HEVM) and is expressed on activated T cells, functioning as negative regulator of T cell immune response through the BTLA-HVEM complex.
Human embryonic kidney cells (HEK 293T) stably expressing this exogenous human BTLA gene are primarily used for drug screening and biological assays.
B and T lymphocyte (BTLA) is also known as BTLA1 or CD272.
Recombinant Cell Line Generation
This 293T human BTLA cell line was generated using pLVX-puro vector with a human BTLA gene sequence.
Cell Line Characterization
Recombinant Cell Line Profile
|Size:||1 Vial (1M Cells)|
|Cell Line ID:||293T/BTLA|
|Cell Type:||Human embryonic kidney|
|Gene ID/Accession #:||NM_181780.3|
|Selection Marker:||Puromycin (0.5ug/ml)|
|Application:||Drug screening and biological assays|
|Morphology:||Fibroblastoid cells growing adherently as monolayer|
|Medium:||DMEM + 10% FBS|
Seed out at ca. 2-3 x 106 cells/80 cm2; split confluent culture 1:4 - 1:5 every 2-3 days
37 °C with 5% CO2
|storage:||Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO|
|Doubling Time:||24-30 hours|
|References:||Nat. Immunol. 4:670-679(2003)|
Thaw the vial as soon as possible upon receipt to insure its viability.
1. Thaw the frozen vial in a 37°C water bath for 1-2 minutes.
2. Transfer the vial into biosafety cabinet. Wipe the surface with 70% ethanol and drop the cell suspension gently into a centrifuge tube containing 9.0 mL complete culture medium.
3. Spin at ~ 125 x g, for 5- 7 minutes at room temperature.
4. Resuspend cell pellet with the appropriate volume of complete medium and transfer the cell suspension into a T25 culture flask.
5. Incubate the culture at 37°, 5% CO2 incubator or refer to the specific culture condition, if possible.
Cell Line Limited Use License
FOR IN VITRO REASEARCH USE ONLY. NOT FOR THERAPEUTIC OR DIAGNOSTIC USE. BY ACCEPTING THIS PRODUCT, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT.
This product may not be resold or transferred by the recipient and may be used only by the recipient and its affiliate (as defined below), in their facility and only for (1) research use, (2) drug discovery and development of biologic drugs, (3) quality assurance testing of biologic drugs, and (4) assays for biologic drugs. No other use, especially any commercial use, is allowed. “Commercial use” means any and all uses of this product by recipient for monetary or other consideration, including providing a service, supplying information or data to unaffiliated third parties, and resale of this product for any use. Recipient has no right to modify, derivatize, genetically engineer, reverse engineer, duplicate or otherwise create variations of the Cells.
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