B7X Cell Line (Monkey) Description
B7X belongs to the B7 costimulatory protein family. Proteins in this family are present on the surface of antigen-presenting cells and interact with ligand bound to receptors on the surface of T cells. Studies have shown that high levels of the encoded protein has been correlated with tumor progression. A pseudogene of this gene is located on chromosome 20. Multiple transcript variants encoding different isoforms have been found for this gene.
Recombinant Cell Line Generation
293T Cynomolgus monkey B7X cell Line was generated using lentiviral vector expressing monkey B7X plus flag sequence.
Cell Line Characterization
Recombinant Cell Line Profile
|Size:||1 Vial (1M Cells)|
|Cell Line ID:||293T/Monkey B7X|
|Cell Type:||Human embryonic kidney|
|Gene ID/Accession #:||XM_015430437.1|
|Selection Marker:||Puromycin (0.5ug/ml)|
|Application:||Drug screening and biological assays|
|Morphology:||Fibroblastoid cells growing adherently as monolayer|
|Medium:||DMEM + 10% FBS|
Seed out at ca. 2-3 x 106 cells/80 cm2; split confluent culture 1:4 - 1:5 every 2-3 days
37 °C with 5% CO2
|storage:||Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO|
|Doubling Time:||24-30 hours|
|References:||Exp. Cell Res. 306:128-141(2005)|
Thaw the vial as soon as possible upon receipt to insure its viability.
1. Thaw the frozen vial in a 37°C water bath for 1-2 minutes.
2. Transfer the vial into biosafety cabinet. Wipe the surface with 70% ethanol and drop the cell suspension gently into a centrifuge tube containing 9.0 mL complete culture medium.
3. Spin at ~ 125 x g, for 5- 7 minutes at room temperature.
4. Resuspend cell pellet with the appropriate volume of complete medium and transfer the cell suspension into a T25 culture flask.
5. Incubate the culture at 37°, 5% CO2 incubator or refer to the specific culture condition, if possible.
Cell Line Limited Use License
FOR IN VITRO REASEARCH USE ONLY. NOT FOR THERAPEUTIC OR DIAGNOSTIC USE. BY ACCEPTING THIS PRODUCT, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT.
This product may not be resold or transferred by the recipient and may be used only by the recipient and its affiliate (as defined below), in their facility and only for (1) research use, (2) drug discovery and development of biologic drugs, (3) quality assurance testing of biologic drugs, and (4) assays for biologic drugs. No other use, especially any commercial use, is allowed. “Commercial use” means any and all uses of this product by recipient for monetary or other consideration, including providing a service, supplying information or data to unaffiliated third parties, and resale of this product for any use. Recipient has no right to modify, derivatize, genetically engineer, reverse engineer, duplicate or otherwise create variations of the Cells.
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To obtain a COMMERCIAL USE License please contact Crown Bioscience, Inc.