Description
The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex.
Western blot analysis of extracts of 293 cells, using Phospho-AMPKβ1-S108 antibody (CB114) at 1:1000 dilution.; Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.; Lysates/proteins: 25ug per lane.; Blocking buffer: 3% BSA.
Product Details
| Synonyms | PRKAB1; AMPK; HAMPKb; 5'-AMP-activated protein kinase subunit beta-1 | ||
|---|---|---|---|
| Host | Rabbit | ||
| IsoType | IgG | ||
| Immunogen Species | Human | ||
| Immunogen | A synthetic phosphorylated peptide around S108 of human AMPKβ1 (NP_006244.2). | ||
| Reactivity | Human | ||
| Tested applications | Human | ||
| Observed MW | 48kDa | ||
| GeneID | 5564 | ||
| Swiss Prot | Q9Y478 | ||
| Purification Method | Affinity purification | ||
| Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. | ||
| Recommended Dilutions | WB 1:500 – 1:2000 | ||
| Modifications | Phospho S108 | ||
| Conjugate | Unconjugated | ||
| Positive samples | 293 | ||
| Storage Conditions | Store at -20°C. Avoid freeze / thaw cycles. | ||
