Description
ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene.
Western blot analysis of extracts of NIH/3T3 cells, using Phospho-ACLY-S455 antibody (CB130) at 1:2000 dilution. NIH/3T3 cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight.; Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.; Lysates/proteins: 25ug per lane.; Blocking buffer: 3% BSA.; Detection: ECL Basic Kit (RM00020).; Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat pancreas using Phospho-ACLY-S455 antibody (CB130) at dilution of 1:100 (40x lens).
Product Details
| Synonyms | ACLY; ACL; ATPCL; CLATP; ATP citrate lyase | ||
|---|---|---|---|
| Host | Rabbit | ||
| IsoType | IgG | ||
| Immunogen Species | Human | ||
| Immunogen | A synthetic phosphorylated peptide around S455 of human ACLY (NP_001087.2). | ||
| Reactivity | Mouse, Rat | ||
| Tested applications | Human | ||
| Observed MW | 121kDa | ||
| GeneID | 47 | ||
| Swiss Prot | P53396 | ||
| Purification Method | Affinity purification | ||
| Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. | ||
| Recommended Dilutions | WB 1:500 – 1:2000 | ||
| Modifications | Phospho S455 | ||
| Conjugate | Unconjugated | ||
| Positive samples | NIH/3T3 | ||
| Cellular Location | Cytoplasm, | ||
| Storage Conditions | Store at -20°C. Avoid freeze / thaw cycles. | ||
