Description
This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13.
Western blot analysis of extracts of various cell lines, using β-Tubulin antibody (CB023) at 1:5000 dilution.; Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.; Lysates/proteins: 25ug per lane.; Blocking buffer: 3% nonfat dry milk in TBST.; Detection: ECL Basic Kit (RM00020).; Exposure time: 10s.
Product Details
| Synonyms | TUBB; CDCBM6; CSCSC1; M40; OK/SW-cl.56; TUBB1; TUBB5; tubulin beta chain | ||
|---|---|---|---|
| Host | Mouse | ||
| IsoType | IgG | ||
| Immunogen Species | Human | ||
| Immunogen | Recombinant protein of human β-Tubulin | ||
| Reactivity | Human, Mouse, Rat, Hamster, Monkey, Goat, Chlamydomonas Reinhardtii | ||
| Tested applications | Human | ||
| Observed MW | 49kDa | ||
| GeneID | 203068 | ||
| Swiss Prot | P07437 | ||
| Purification Method | Affinity purification | ||
| Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. | ||
| Recommended Dilutions | WB 1:5000 – 1:10000 | ||
| Modifications | Unmodified | ||
| Conjugate | Unconjugated | ||
| Positive samples | SH-SY5Y,MCF-7,HepG2,HeLa,Mouse brain,Mouse lung,Rat brain | ||
| Cellular Location | Cytoplasm,cytoskeleton, | ||
| Storage Conditions | Store at -20°C. Avoid freeze / thaw cycles. | ||
