The IL2 receptor (IL2R) exists in three alternative forms made up from the individual components of CD25, CD122, and CD132. CD25 represents the low affinity alpha chain of the IL2R, a type I transmembrane glycoprotein containing two CCP domains. It is rich in O linked carbohydrates and has a short cytoplasmic tail. CD25 is expressed on activated T cells, B cells, NK cells and monocytes of all mouse strains tested. It is transiently expressed at a low level during B cell development in bone marrow on the CD45R/B220 low TdT- sIg- pre-B II and CD45R/B220low TdT- sIgM+sIgD- immature B cells, but not on the CD45R/B220 low TdT+ sIg- pro-B/pre B I stage nor on CD45R/B220high TdT- sIgM+sIgD+ mature B cells. CD25 is expressed at a higher level during very early T cell development in fetal and adult thymus. CD25 expression is a common denominator of a specific immunomodulatory B-cell subset ready to proliferate upon IL-2 stimulation.
|Immunogen:||Recombinant full length (1-272 aa) of human CD25|
|Synonyms:||IL2RA; CD25; IDDM10; IL2R; TCGFR|
|Observed MW:||Refer to figure|
|Specificity:||This antibody recognizes CD25 protein|
IHC: 2-5 ug/ml
WB: 0.1-1 ug/ml
|Purification Method:||Affinity purified|
|Buffer:||Sterile PBS (pH7.2) containing antibody stabilizer|
|Storage Conditions:||Stable for 1 year at -20.0°C from date of receipt.|
FOR RESEARCH USE ONLY. NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE.