CD40 ligand (CD40L), also known as CD154, is a cytokine that binds to CD40/TNFRSF5, and costimulates T-cell proliferation and cytokine production. Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation. Induces the activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells. Induces tyrosine phosphorylation of isoform 3 of CD28. Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4. Involved in immunoglobulin class switching. This CD40L antibody was developed from mouse immunization, and expressed in adherent culture of mouse hybridoma clone CB37.
|Lot:||Please refer to product vial|
|Immunogen:||Human CD40L, ECD domain|
|Synonyms:||CD40 ligand, CD40LG, CD154|
|Applications:||ELISA, FACS, WB|
ELISA: 0.2-1.0 ug/mL, FACS: 1-5 ug/mL, WB: 1-5 ug/mL
|QC:||Endotoxin level: <2 EU/mg by the LAL method, Purity: >90% by SDS-PAGE|
|Production Method:||Expressed from mouse hybridoma clone CB37|
|Purification Method:||Affinity purified|
|Buffer:||PBS, pH 7.4|
|Storage Conditions:||Store at -20°C. Avoid freeze / thaw cycles. Stable for 12 months from date of purchase when stored as recommended.|
FOR RESEARCH USE ONLY. NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE.
100 ug - $325.00 – $485.00