Appropriate pairs of anti-CD2 antibodies will stimulate peripheral T-cell proliferation and effector function.1 The CD2 molecule has been shown to be the receptor for leukocyte function antigen (LFA-3/CD58),2 the binding of which augments T-cell activation mediated by the T-cell antigen receptor (TCR).3 Anti-Human CD2 can be used to deplete CD2 positive cells by complement-mediated cytotoxicity. Analyses of NK lymphocyte subsets and identification of lymphomas and leukemias of T-cell origin have been done using Anti-CD2 antibodies.
|Ig Subclass||Mouse IgG2a|
Mouse Anti-Human CD2 (Clone G11) recognizes Human CD2. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Anti-Human CD2 recognizes a cell surface glycoprotein (Mr 45-59 kDa) on human lymphocytes. This antigen also forms the binding site for sheep erythrocytes.
|Antigen Distribution||The CD2 antigen is present on approximately 80% of normal peripheral blood lymphocytes, >95% of thymocytes, 100% of mature circulating T-cells and a subset of NK cells.|
|Reported Molecule||Fluorescein (FITC) is a small fluorescent organic molecule with a molecular weight of 473 daltons with an absorbance maximum of ~492 nm and an emission maximum of ~518 nm.|
|Formulation||This Fluorescein (FITC) conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.|
|Dilution||Each investigator should determine their own optimal working dilution for specific applications.|
FOR RESEARCH USE ONLY. NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE
$50.00 – $95.00