Our Isotype Control Antibodies are the Ideal Research Controls

CrownBio Life Science is a newly launched division of the company specializing in in vivo and in vitro research reagents to support our partners’ preclinical research efforts. The Life Science Division of CrownBio is headquartered in Santa Clara, CA, and now provides a range of research reagents including isotype control antibodies.

In Vivo Grade Isotype Control Antibodies

  • Suitable for use in in vivo studies as well as functional studies, WB, IHC, FC, IP etc
  • Very low endotoxin levels (< 0.5 EU/mg)
  • Supplied at high concentration (3-5 mg/ml)
  • Supplied in PBS only; no carriers or additives
  • Purity of > 95% as analyzed by SDS-PAGE and HPLC

Isotype controls are essential for validating experimental results. They are commonly used in in vivo antibody studies and immunological assays as a negative control. Isotype controls lack specificity to the experimental target of interest, but will exhibit any of the same non-specific binding that may arise from non-CDR antibody elements of the target-specific primary antibody. The isotype control should match the target-specific primary antibody′s host species, isotype (including subclass and light chain), level of conjugation, and IgG concentration.

Crown Bioscience has developed human and mouse IgG isotype controls specifically for pharma and biotechnology companies with a need for in vivo grade antibody controls. These isotype controls have been developed and formulated specifically for use in in vivo animal studies and high throughput screening assays. Unlike standard isotype control antibodies, Crown Bioscience’s isotype control antibodies have been produced in a low endotoxin controlled environment and are QC′d for both endotoxin levels and IgG purity. They are supplied at high concentration without any additives. Our isotype controls have been successfully validated and used by several pharmaceutical and biotech companies in their in vivo efficacy studies.

List Of Currently Available In Vivo Grade Isotype Control Antibodies

Product Name Catalog #
Species Details
Human IgG1, kappa Isotype Control C0001 CB1

Details

Human IgG1 (D265A), kappa Isotype Control C0020 CB13

Details

Human IgG1 (non-glycosylated), kappa Isotype Control C0019 CB12

Details

Human IgG2, kappa Isotype Control C0002 CB2

Details

Human IgG3, kappa Isotype Control C0003 CB3

Details

Human IgG4, kappa Isotype Control C0004 CB4

Details

Product Name Catalog #
Species Details
Mouse IgG1, kappa Isotype Control C0005 CB5

Details

Mouse IgG2a, kappa Isotype Control C0006 CB6

Details

Mouse IgG2a (non-glycosylated), kappa Isotype Control C0007 CB7

Details

Mouse IgG2b, kappa Isotype Control C0008 CB8

Details

Mouse IgG3, kappa Isotype Control C0009 CB9

Details

Product Name Catalog #
Species Details
Rabbit IgG, kappa Isotype Control C0012 CB16

Details

Product Name Catalog #
Species Details
Rat IgG1, kappa Isotype Control C0010 CB14

Details

Rat IgG2a, kappa Isotype Control C0011 CB15

Details

These human and mouse IgG isotype controls were raised against HEL (hen egg lysozyme), a target that does not exist in mouse or human, in order to avoid cross reactivity. Each isotype control was expressed in mammalian cells and purified with a protein A affinity column. Each isotype control is suitable for use as a negative control, standard, blocking agent or coating protein in various applications.

Bulk quantities of these antibodies are readily available. Please contact us at 855.827.6968 or products@crownbio.com for a quote.

Figure 1. Representative QC data: SEC-HPLC (0.5 ml/min; 30 min; UV280) analysis of Human IgG1, kappa Isotype Control, catalog # C0001.

Figure 2. Representative QC data: SDS-PAGE (12% SDS-Polyacrylamide gel) analysis of Human IgG1, kappa Isotype Control,catalog # C0001, under reducing and non-reducing conditions.

Contact

To receive pricing or for any product related questions contact us at:

Crown Bioscience Inc. Life Science Division
3375 Scott Blvd., Suite 108, Santa Clara, CA 95054

Tel: 855.827.6968 Email: products@crownbio.com
Fax: 408.884.2339 Ordering Terms & Conditions

Ask a Question / Request a Quote

For large volume quotes, or to ask a question about this product please complete the form below, or call:

+1 855 827 6968
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