The Most Comprehensive MAPK Mutation Panel On the Market

The MAPK signaling pathway encompasses a series of signal transduction events that begin with the engagement of EGFR at the outer cell membrane, and proceed through KRAS, BRAF, MEK, and ERK. This signaling cascade ultimately results in transcriptional activation of key genes that promote cellular proliferation, survival, differentiation, motility, and angiogenesis. As such, the MAPK pathway is one of the most frequently activated pathways in cancer, and several drugs have pharmacogenomics profiles that depend upon the MAPK mutational status.

CrownBio now offers a series of isogenic cell lines featuring diverse genetic mutations in EGFR, KRAS, and BRAF. Isogenic cell lines represent a unique toolset for studying the impact of genotype on cellular phenotype. An isogenic cell line is a cell line that has been engineered from a parental line through the introduction of a targeted gene mutation. In doing so, the parental cell line inherently becomes a control line to which the engineered line can be referenced. Isogenic cell line pairs can be incredibly useful as tools for studying cellular biology, such as the impact of genotype on cellular phenotype, as well as for high throughput screening to identify lead compounds for cancer therapeutics.

These isogenic cell lines are produced in either RKO or HCT116 cell lines. Matched parental RKO and HCT116 cell lines are also available for purchase.

Related reference standards, including genomic DNA, FFPE blocks and cell pellets are available too.

Product Name Catalog # Cosmic ID Details
BRAF V600D Cell Line C8048C COSM477

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BRAF V600E Cell Line (c.1799_1800TG>AA) C8046C COSM475

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BRAF V600E Cell Line (c.1799T>A) C8047C COSM476

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BRAF V600G Cell Line C8050C COSM6137

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BRAF V600K Cell Line C8044C COSM473

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BRAF V600M Cell Line C8049C COSM1130

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BRAF V600R Cell Line C8045C COSM474

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EGFR V769_D770insASV Cell Line C8026C COSM12376

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EGFR c.2310_2311insGGT p.D770_N771insG Cell Line C8027C COSM12378

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EGFR c.2319_2320insCAC p.H773_V774insH Cell Line C8028C COSM12377

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EGFR E746_A750>IP Cell Line C8024C 13550

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EGFR E746_A750del Cell Line (c.2235_2249del15) C8016C COSM6223

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EGFR E746_A750del Cell Line (c.2236_2250del15) C8017C 6225

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EGFR E746_S752>A Cell Line C8007C COSM12367

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EGFR E746_S752>D Cell Line C8005C COSM6220

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EGFR E746_S752>V Cell Line C8008C COSM12384

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EGFR E746_T751>A Cell Line C8010C COSM12678

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EGFR E746_T751>I Cell Line C8014C COSM13551

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EGFR E746_T751>V Cell Line C8013C 12386

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EGFR E746_T751del Cell Line C8009C COSM12728

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EGFR G719A Cell Line C8002C COSM6239

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EGFR G719C Cell Line C8001C COSM6253

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EGFR G719S Cell Line C8000C 6252

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EGFR K745_E749del Cell Line C8015C COSM26038

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EGFR L747_A750>P Cell Line (c.2238_2248>GC) C8022C COSM12422

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EGFR L747_A750>P Cell Line (c.2239_2248TTAAGAGAAG>C) C8021C COSM12382

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EGFR L747_E749del Cell Line C8023C COSM6218

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EGFR L747_P753>Q Cell Line C8006C COSM12387

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EGFR L747_P753>S Cell Line C8004C COSM12370

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EGFR L747_S752del Cell Line C8003C COSM6255

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EGFR L747_T751>P Cell Line C8018C COSM12383

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EGFR L747_T751>Q Cell Line C8020C COSM12419

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EGFR L747_T751>S Cell Line C8019C COSM6210

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EGFR L747_T751del Cell Line (c.2239_2253del15) C8011C 6254

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EGFR L747_T751del Cell Line (c.2240_2254del15) C8012C 12369

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EGFR L858R Cell Line C8030C COSM6224

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EGFR L861Q Cell Line C8031C COSM6213

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EGFR S768I Cell Line C8025C COSM6241

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EGFR T790M Cell Line C8029C COSM6240

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HCT116 Cell Line (Wild Type) C8052C-WT

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HRAS G13R Cell Line C6078C 486

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HRAS Q61K Cell Line C6079C 496

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HRAS Q61R Cell Line C6080C 487

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KRAS G12A Cell Line C8037C COSM522

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KRAS G12C Cell Line C8032C COSM516

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KRAS G12D Cell Line C8036C 521

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KRAS G12R Cell Line C8034C 518

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KRAS G12S Cell Line C8033C 517

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KRAS G12V Cell Line C8035C 520

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KRAS G13A Cell Line C8042C COSM533

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KRAS G13C Cell Line C8038C COSM527

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KRAS G13D Cell Line C8041C 532

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KRAS G13R Cell Line C8040C COSM529

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KRAS G13S Cell Line C8039C COSM528

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KRAS G13V Cell Line C8043C COSM534

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KRAS Q61H Cell Line C6062C 554

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NRAS E62fs*6 Cell Line C6200C 5044298

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NRAS G12C Cell Line C6067C 562

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NRAS G12D Cell Line C6065C 564

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NRAS G12S Cell Line C6066C 563

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NRAS G13R Cell Line C6069C 569

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NRAS Q61K Cell Line C6072C 580

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NRAS Q61R Cell Line (c.182A>G) C6071C 584

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NRAS Q61R Cell Line (c.182A>T) C6073C 583

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PIK3CA E542K Cell Line C6053C 760

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PIK3CA E545G Cell Line C6055C 764

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PIK3CA E545K Cell Line C6054C 763

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PIK3CA H1047R Cell Line C6051C 775

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PIK3CA Q546K Cell Line C6057C 766

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RKO Cell Line (Wild Type) C8051C-WT

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TP53 G245C Cell Line C6090C 11081

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TP53 G245D Cell Line C6088C 43606

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TP53 G245S Cell Line C6087C 6932

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TP53 G245V Cell Line C6089C 11196

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