The Most Comprehensive MAPK Mutation Panel On the Market, And More

The MAPK signaling pathway encompasses a series of signal transduction events that begin with the engagement of EGFR at the outer cell membrane, and proceed through KRAS, BRAF, MEK, and ERK. This signaling cascade ultimately results in transcriptional activation of key genes that promote cellular proliferation, survival, differentiation, motility, and angiogenesis. As such, the MAPK pathway is one of the most frequently activated pathways in cancer, and several drugs have pharmacogenomics profiles that depend upon the MAPK mutational status.

CrownBio now offers a series of isogenic cell lines featuring diverse genetic mutations in MAPK-related targets, and genes involved in DNA repair, Wnt signaling, Cell adhesion, metabolism, and more. Isogenic cell lines represent a unique toolset for studying the impact of genotype on cellular phenotype. An isogenic cell line is a cell line that has been engineered from a parental line through the introduction of a targeted gene mutation. In doing so, the parental cell line inherently becomes a control line to which the engineered line can be referenced. Isogenic cell line pairs can be incredibly useful as tools for studying cellular biology, such as the impact of genotype on cellular phenotype, as well as for high throughput screening to identify lead compounds for cancer therapeutics.

These isogenic cell lines are produced in either RKO or HCT116 cell lines. Matched parental RKO and HCT116 cell lines are also available for purchase.

Related reference standards, including genomic DNA, FFPE blocks and cell pellets are available too.

Name Catalog # Price  

APC E1309fs*4 Cell Line

C6146C
Clear

APC R213* Cell Line

C6150C
Clear

BRAF V600D Cell Line

C8048C
Clear

BRAF V600E Cell Line (c.1799_1800TG>AA)

C8046C
Clear

BRAF V600E Cell Line (c.1799T>A)

C8047C
Clear

BRAF V600G Cell Line

C8050C
Clear

BRAF V600K Cell Line

C8044C
Clear

BRAF V600M Cell Line

C8049C
Clear

BRAF V600R Cell Line

C8045C
Clear

BRCA1 E1161Fs Cell Line

C6112C
Clear

BRCA1 E23fs*17 Cell Line

C6107C
Clear

BRCA1 Q1756fs*74 Cell Line

C6108C
Clear

CTNNB1 S33C Cell Line

C6139C
Clear

CTNNB1 S33Y Cell Line

C6140C
Clear

CTNNB1 S45del Cell Line

C6143C
Clear

EGFR c.2310_2311insGGT p.D770_N771insG Cell Line

C8027C
Clear

EGFR c.2319_2320insCAC p.H773_V774insH Cell Line

C8028C
Clear

EGFR C797S/T790M Cell Line (c.2389T>A)

C6177C
Clear

EGFR C797S/T790M Cell Line (c.2390G>C)

C6176C
Clear

EGFR E746_A750>IP Cell Line

C8024C
Clear

EGFR E746_A750del Cell Line (c.2235_2249del15)

C8016C
Clear

EGFR E746_A750del Cell Line (c.2236_2250del15)

C8017C
Clear

EGFR E746_S752>A Cell Line

C8007C
Clear

EGFR E746_S752>D Cell Line

C8005C
Clear

EGFR E746_S752>V Cell Line

C8008C
Clear

EGFR E746_T751>A Cell Line

C8010C
Clear

EGFR E746_T751>I Cell Line

C8014C
Clear

EGFR E746_T751>V Cell Line

C8013C
Clear

EGFR E746_T751del Cell Line

C8009C
Clear

EGFR G719A Cell Line

C8002C
Clear

EGFR G719C Cell Line

C8001C
Clear

EGFR G719S Cell Line

C8000C
Clear

EGFR K745_E749del Cell Line

C8015C
Clear

EGFR L747_A750>P Cell Line (c.2238_2248>GC)

C8022C
Clear

EGFR L747_A750>P Cell Line (c.2239_2248TTAAGAGAAG>C)

C8021C
Clear

EGFR L747_E749del Cell Line

C8023C
Clear

EGFR L747_P753>Q Cell Line

C8006C
Clear

EGFR L747_P753>S Cell Line

C8004C
Clear

EGFR L747_S752del Cell Line

C8003C
Clear

EGFR L747_T751>P Cell Line

C8018C
Clear

EGFR L747_T751>Q Cell Line

C8020C
Clear

EGFR L747_T751>S Cell Line

C8019C
Clear

EGFR L747_T751del Cell Line (c.2239_2253del15)

C8011C
Clear

EGFR L747_T751del Cell Line (c.2240_2254del15)

C8012C
Clear

EGFR L858R Cell Line

C8030C
Clear

EGFR L861Q Cell Line

C8031C
Clear

EGFR Q787R Cell Line

C6182C
Clear

EGFR S768I Cell Line

C8025C
Clear

EGFR T790M Cell Line

C8029C
Clear

EGFR V769_D770insASV Cell Line

C8026C
Clear

EGFR V843I Cell Line

C6185C
Clear

ESR1 D538G Cell Line

C6169C
Clear

ESR1 Y537C Cell Line

C6172C
Clear

ESR1 Y537N Cell Line

C6171C
Clear

ESR1 Y537S Cell Line

C6170C
Clear

FLT3 D835H Cell Line

C6154C
Clear

FLT3 I836delI Cell Line

C6157C
Clear

HCT116 Cell Line (Wild Type)

C8052C-WT
Clear

HRAS G13R Cell Line

C6078C
Clear

HRAS Q61K Cell Line

C6079C
Clear

HRAS Q61R Cell Line

C6080C
Clear

IDH2 R140L Cell Line

C6227C
Clear

IDH2 R140Q Cell Line

C6225C
Clear

IDH2 R140W Cell Line

C6226C
Clear

IDH2 R172G Cell Line

C6232C
Clear

IDH2 R172K Cell Line

C6228C
Clear

IDH2 R172M Cell Line

C6229C
Clear

IDH2 R172S Cell Line

C6230C
Clear

IDH2 R172W Cell Line

C6231C
Clear

KRAS G12A Cell Line

C8037C
Clear

KRAS G12C Cell Line

C8032C
Clear

KRAS G12D Cell Line

C8036C
Clear

KRAS G12R Cell Line

C8034C
Clear

KRAS G12S Cell Line

C8033C
Clear

KRAS G12V Cell Line

C8035C
Clear

KRAS G13A Cell Line

C8042C
Clear

KRAS G13C Cell Line

C8038C
Clear

KRAS G13D Cell Line

C8041C
Clear

KRAS G13R Cell Line

C8040C
Clear

KRAS G13S Cell Line

C8039C
Clear

KRAS G13V Cell Line

C8043C
Clear

KRAS Q61H Cell Line

C6062C
Clear

NRAS E62fs*6 Cell Line

C6200C
Clear

NRAS G12C Cell Line

C6067C
Clear

NRAS G12D Cell Line

C6065C
Clear

NRAS G12S Cell Line

C6066C
Clear

NRAS G13R Cell Line

C6069C
Clear

NRAS Q61K Cell Line

C6072C
Clear

NRAS Q61R Cell Line (c.182A>G)

C6071C
Clear

NRAS Q61R Cell Line (c.182A>T)

C6073C
Clear

PIK3CA C420R Cell Line

C6060C
Clear

PIK3CA E542K Cell Line

C6053C
Clear

PIK3CA E545G Cell Line

C6055C
Clear

PIK3CA E545K Cell Line

C6054C
Clear

PIK3CA G13V Cell Line

C6070C
Clear

PIK3CA H1047L Cell Line

C6052C
Clear

PIK3CA H1047R Cell Line

C6051C
Clear

PIK3CA Q546K Cell Line

C6057C
Clear

PTEN R130G Cell Line

C6127C
Clear

PTEN R130Q Cell Line

C6128C
Clear

PTEN R233* Cell Line

C6133C
Clear

RKO Cell Line (Wild Type)

C8051C-WT
Clear

TP53 C242Y Cell Line

C6086C
Clear

TP53 D281G Cell Line

C6101C
Clear

TP53 G245C Cell Line

C6090C
Clear

TP53 G245D Cell Line

C6088C
Clear

TP53 G245S Cell Line

C6087C
Clear

TP53 G245V Cell Line

C6089C
Clear

TP53 R158C Cell Line

C6193C
Clear

TP53 R158G Cell Line

C6194C
Clear

TP53 R248L Cell Line

C6093C
Clear

TP53 R248Q Cell Line

C6091C
Clear

TP53 R248W Cell Line

C6092C
Clear

TP53 R273C Cell Line

C6099C
Clear

TP53 R273H Cell Line

C6098C
Clear

TP53 R282G Cell Line

C6103C
Clear

TP53 R282Q Cell Line

C6104C
Clear