Analyze the Impact of Workflow Changes Without Using Your Precious Samples

CrownBio now offers cell pellet reference standards, which represent biologically-relevant controls that can be directly incorporated into your sample processing workflows in order to optimize protocols, evaluate assay sensitivity and specificity, and analyze the impact of workflow changes on downstream analysis. These frozen cell pellets can be paired with our FFPE cell blocks to evaluate the impact of formalin fixation on DNA integrity and assay results.

These MAPK cell pellet reference standards are derived from isogenic MAPK mutation cell lines with various recurrent pathway-activating mutations in MAPK-related genes, based on data from the Sanger Institute’s COSMIC database.

Product Name Catalog # Cosmic ID Details
BRAF V600D Cell Pellet C7048P COSM477

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BRAF V600E Cell Pellet (c.1799_1800TG>AA) C7046P COSM475

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BRAF V600E Cell Pellet (c.1799T>A) C7047P COSM476

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BRAF V600G Cell Pellet C7050P COSM6137

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BRAF V600K Cell Pellet C7044P COSM473

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BRAF V600M Cell Pellet C7049P COSM1130

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BRAF V600R Cell Pellet C7045P COSM474

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EGFR V769_D770insASV Cell Pellet C7026P COSM12376

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EGFR c.2310_2311insGGT p.D770_N771insG Cell Pellet C7027P COSM12378

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EGFR c.2319_2320insCAC p.H773_V774insH Cell Pellet C7028P COSM12377

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EGFR E746_A750>IP Cell Pellet C7024P COSM13550

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EGFR E746_A750del Cell Pellet (c.2235_2249del15) C7016P COSM6223

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EGFR E746_A750del Cell Pellet (c.2236_2250del15) C7017P COSM6225

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EGFR E746_S752>A Cell Pellet C7007P COSM12367

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EGFR E746_S752>D Cell Pellet C7005P COSM6220

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EGFR E746_S752>V Cell Pellet C7008P COSM12384

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EGFR E746_T751>A Cell Pellet C7010P COSM12678

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EGFR E746_T751>I Cell Pellet C7014P COSM13551

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EGFR E746_T751>V Cell Pellet C7013P COSM12386

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EGFR E746_T751del Cell Pellet C7009P COSM12728

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EGFR G719A Cell Pellet C7002P COSM6239

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EGFR G719C Cell Pellet C7001P COSM6253

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EGFR G719S Cell Pellet C7000P COSM6252

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EGFR K745_E749del Cell Pellet C7015P COSM26038

Details

EGFR L747_A750>P Cell Pellet (c.2238_2248>GC) C7022P COSM12422

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EGFR L747_A750>P Cell Pellet (c.2239_2248TTAAGAGAAG>C) C7021P COSM12382

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EGFR L747_E749del Cell Pellet C7023P COSM6218

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EGFR L747_P753>Q Cell Pellet C7006P COSM12387

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EGFR L747_P753>S Cell Pellet C7004P COSM12370

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EGFR L747_S752del Cell Pellet C7003P COSM6255

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EGFR L747_T751>P Cell Pellet C7018P COSM12383

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EGFR L747_T751>Q Cell Pellet C7020P COSM12419

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EGFR L747_T751>S Cell Pellet C7019P COSM6210

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EGFR L747_T751del Cell Pellet (c.2239_2253del15) C7011P COSM6254

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EGFR L747_T751del Cell Pellet (c.2240_2254del15) C7012P COSM12369

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EGFR L858R Cell Pellet C7030P COSM6224

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EGFR L861Q Cell Pellet C7031P COSM6213

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EGFR S768I Cell Pellet C7025P COSM6241

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EGFR T790M Cell Pellet C7029P COSM6240

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HCT116 Cell Pellet (Wild Type) C7052P-WT

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HRAS G13R Cell Pellet C6078P 486

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HRAS Q61K Cell Pellet C6079P 496

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HRAS Q61R Cell Pellet C6080P 487

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KRAS G12A Cell Pellet C7037P COSM522

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KRAS G12C Cell Pellet C7032P COSM516

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KRAS G12D Cell Pellet C7036P COSM521

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KRAS G12R Cell Pellet C7034P COSM518

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KRAS G12S Cell Pellet C7033P COSM517

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KRAS G12V Cell Pellet C7035P COSM520

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KRAS G13A Cell Pellet C7042P COSM533

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KRAS G13C Cell Pellet C7038P COSM527

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KRAS G13D Cell Pellet C7041P COSM532

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KRAS G13R Cell Pellet C7040P COSM529

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KRAS G13S Cell Pellet C7039P COSM528

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KRAS G13V Cell Pellet C7043P COSM534

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KRAS Q61H Cell Pellet C6062P 554

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NRAS E62fs*6 Cell Pellet C6200P 5044298

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NRAS G12C Cell Pellet C6067P 562

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NRAS G12D Cell Pellet C6065P 564

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NRAS G12S Cell Pellet C6066P 563

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NRAS G13R Cell Pellet C6069P 569

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NRAS Q61K Cell Pellet C6072P 580

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NRAS Q61R Cell Pellet (c.182A>G) C6071P 584

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NRAS Q61R Cell Pellet (c.182A>T) C6073P 583

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PIK3CA E542K Cell Pellet C6053P 760

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PIK3CA E545G Cell Pellet C6055P 764

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PIK3CA E545K Cell Pellet C6054P 763

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PIK3CA H1047R Cell Pellet C6051P 775

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PIK3CA Q546K Cell Pellet C6057P 766

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RKO Cell Pellet (Wild Type) C7051P-WT

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TP53 G245C Cell Pellet C6090P 11081

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TP53 G245D Cell Pellet C6088P 43606

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TP53 G245S Cell Pellet C6087P 6932

Details

TP53 G245V Cell Pellet C6089P 11196

Details

Related isogenic cell lines and additional reference standards, including FFPE blocks and genomic DNA are available too.

About the MAPK Pathway

The MAPK signaling pathway encompasses a series of signal transduction events that begin with the engagement of EGFR at the outer cell membrane, and proceed through KRAS, BRAF, MEK, and ERK. This signaling cascade ultimately results in transcriptional activation of key genes that promote cellular proliferation, survival, differentiation, motility, and angiogenesis. As such, the MAPK pathway is one of the most frequently activated pathways in cancer, and several drugs have pharmacogenomics profiles that depend upon the MAPK mutational status.

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