Recombinant Cell Lines for Drug Discovery and Development

Crown Bioscience has developed several stable cell lines for drug discovery and development that cover important targets or pathways in oncology.

List of currently available cell lines:

Recombinant Cell Lines

Product Name Catalog #
Species Details
B7X Cell Line (Human) C2065 Human

Details

B7X Cell Line (Mouse) C2073 Human

Details

B7X Cell Line (Monkey) C2074 Human

Details

BTLA Cell Line C2044 Human

Details

CD137 Cell Line C2012 Human

Details

CD154 Cell Line C2041 Human

Details

CD160 Cell Line C2033 Human

Details

CD226 Cell Line C2023 Human

Details

CD258 Cell Line C2040 Human

Details

CD269 Cell Line C2045 Human

Details

CD27 Cell Line C2027 Human

Details

CD276 Cell Line C2024 Human

Details

CD28 Cell Line C2017 Human

Details

CD30 Cell Line C2028 Human

Details

CD30L Cell Line C2022 Human

Details

CD40 Cell Line C2029 Human

Details

CD48 Cell Line C2030 Human

Details

CD73 Cell Line C2018 Human

Details

CD74 Cell Line C2042 Human

Details

CD80 Cell Line C2043 Human

Details

CD86 Cell Line C2031 Human

Details

CEACAM-1 Short Isoform Cell Line C2062 Human

Details

CEACAM-1 Long Isoform Cell Line C2063 Human

Details

CEACAM-5 Cell Line C2064 Human

Details

CTLA-4 Cell Line C2015 Human

Details

GITR Cell Line C2011 Human

Details

GITRL Cell Line C2046 Human

Details

HER2 Cell Line (T47D) C2068 Human

Details

HER2 Cell Line (Ba/F3) C2070 Mouse

Details

HVEM Cell Line C2034 Human

Details

ICOS Cell Line C2016 Human

Details

ICOSLG Cell Line C2019 Human

Details

LAG3 Cell Line C2020 Human

Details

NF-kappa B Reporter Cell Line C2026 Human

Details

OX40 Cell Line C2014 Human

Details

CD244 Cell Line C2048 Human

Details

p95HER2 Cell Line (T47D) C2069 Human

Details

TIM1 Cell Line C2032 Human

Details

p95HER2 Cell Line (Ba/F3) C2071 Mouse

Details

PD-1 Cell Line C2004 Human

Details

SLC34A2-ROS1-G2032R Cell Line C2067 Mouse

Details

PD-L1 Cell Line C2005 Human

Details

PD-L2 Cell Line C2025 Human

Details

RANKL Cell Line C2013 Hamster

Details

SLAMF1 Cell Line C2047 Human

Details

SLC34A2-ROS1 Cell Line C2066 Mouse

Details

TIM3 Cell Line C2021 Human

Details

PD-1 Cell Line Data

FACS analysis of cell surface PD-1 expression by an anti-hPD-1 antibody and isotype control antibody.

PD-1 Cell Line Data

FACS analysis of cell surface PD-1 expression by an anti-hPD-1 antibody and isotype control antibody.

ALK Kinase Fusion Screening Cell Lines

These cell lines are stable Ba/F3 clones expressing exogenous EML4-ALK fusion variants, including wild type and L1196M, F1174L and C1156Y mutations. They are ideal for cell-based screening of potential ALK inhibitors that can overcome Crizotinib resistance.

Product Name Catalog #
Species Details
EML4-ALK L1196M Cell Line C2001 Mouse

Details

EML4-ALK F1174L Cell Line C2002 Mouse

Details

EML4-ALK Cell Line C2000 Mouse

Details

EML4-ALK C1156Y Cell Line C2003 Mouse

Details

EML4 ALK Cell Lines Characterization Data

Characterization of EML4-ALK fusion protein expression in different EML4-ALK stable cell lines by Western blot. Protein lysates from NCI-H2228 cells harboring EML4-ALK fusion, and KARPAS-299 cells harboring NPM1-ALK fusion, were compared side by side in the same blot. Parental Ba/F3 cell line extract was used as negative control.

BCR-ABL Kinase Fusion Screening Cell Lines

Our BCR-ABL (breakpoint cluster region – Abelson murine leukemia viral oncogene homolog 1) cell line is a stable cell line expressing the exogenous BCR-ABL fusion gene with T315I point mutation in the ABL kinase domain, which is present in most cases of chronic myelogenous leukemia (CML). This cell line is ideal for cell-based screening of potential kinase inhibitors.

Product Name Catalog #
Species Details
BCR-ABL Cell Line C2072 Mouse

Details

BCR-ABL T315I Cell Line C2008 Mouse

Details

BCR-ABL Characterization Data

Characterization of drug sensitivity in BCR-ABL T315I Cell Line, catalog # C2008. C2008 was treated with 5 different inhibitors for 72 hours in 96-well plates. Cell viability was measured using CellTiter-Glo. The dose responsive curve and the IC50 of these inhibitors were fitted and calculated using GraphPad Prism.

EGFR Cell Lines

EGFR (epidermal growth factor receptor) is a cell-surface receptor tyrosine kinase. Activation of this receptor has been associated with a number of cancers, including lung and colon cancer. The identification of EGFR as driver gene has led to the development of anti-cancer therapeutic agents, including Gefitinib, Erlotinib, Afatinib, Osimertinib (AZD9291), and Cetuximab. The EGFR T790M mutation causes resistance to the first generation of reversible EGFR inhibitors, including Erlotinib, and the development of new irreversible EGFR inhibitors such as Afatinib and Osimertinib.

Ba/F3, a murine interleukin-3 dependent pro-B cell line, is a popular system for exploring both kinases and their kinase inhibitors, because some protein kinases can render Ba/F3 cells to be independent of IL-3, while their inhibitors antagonize this effect. Our EGFR Cell Lines were generated using pLVX-puro lentivirus system to express EGFR and the EGFR T790M mutation.

Product Name Catalog #
Species Details
EGFR Cell Line C2009 Mouse

Details

EGFR T790M Cell Line C2010 Mouse

Details

EGFR-C797S Cell Line C2051 Mouse

Details

EGFR-D770_N771insSVD Cell Line C2059 Mouse

Details

EGFR-DEL19 Cell Line C2037 Mouse

Details

EGFR-Del19/C797S Cell Line C2049 Mouse

Details

EGFR-Del19/T790M Cell Line C2038 Mouse

Details

EGFR-Del19/T790M/C797S Cell Line C2050 Mouse

Details

EGFR-G719S Cell Line C2035 Mouse

Details

EGFR-G719S/T263P Cell Line C2036 Mouse

Details

EGFR-H773_V774insNPH Cell Line C2058 Mouse

Details

EGFR-L858R Cell Line C2039 Mouse

Details

EGFR-L858R/C797S Cell Line C2052 Mouse

Details

EGFR-L858R/T790M Cell Line C2055 Mouse

Details

EGFR-L858R/T790M/C797S Cell Line C2056 Mouse

Details

EGFR-L861Q Cell Line C2053 Mouse

Details

EGFR-T790M/C797S Cell Line C2054 Mouse

Details

EGFR-T790M/L861Q Cell Line C2057 Mouse

Details

EGFR T790M Cell Line Characterization Data

Characterization of EGFR and its mutants overexpressing in stable cell lines by Western Blot. 1. Parental Ba/F3 cell line showing no EGFR expression, 2. EGFR Wild Type cell line expression, 3. Parental Ba/F3 cell line no EGFR expression and 4. EGFR-T790M mutant cell line expression.

Characterization of drug sensitivity in Ba/F3 EGFR T790M Cell Line, catalog # C2010. C2010 was treated with 4 different inhibitors for 72 hours in 96-well plates. Cell viability was measured using CellTiter-Glo. The dose responsive curve and the IC50 of these inhibitors were fitted and calculated using GraphPad Prism.

WNT Pathway Reporter Cell Line

Our 293T Wnt Luciferase Cell Line provides a convenient tool for screening of inhibitors or activators of Wnt signaling pathway, which is frequently activated abnormally in many human cancers. It is a HEK 293T cell clone stably integrated with Wnt reporter expression cassette with the firefly luciferase gene under the control of promoter containing 14 TCF-binding sites. The Wnt reporter expression is robustly induced by exogenous Wnt stimulation, which can be easily measured by the luciferase activity.

Product Name Catalog #
Species Details
Wnt Luciferase Cell Line C2006 Human

Details

Wnt Luciferase Data

Wnt luciferase cells (C2006) seeded in 96-well plate were stimulated with soluble Wnt3a and R-spondin 2 in the absence and presence of Wnt inhibitory protein LGR5-Fc or DKK1 for 20h, followed by luciferase activity measurement.

Ask a Question / Request a Quote

For large volume quotes, or to ask a question about this product please complete the form below, or call:

+1 855 827 6968
6am – 5pm (PST)